ngf induced tf1 proliferation assay tf1 cells Search Results


a ngf induced tf 1 cell proliferation  (ATCC)
97
ATCC a ngf induced tf 1 cell proliferation
A Ngf Induced Tf 1 Cell Proliferation, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tf1 cells  (DSMZ)
94
DSMZ tf1 cells
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Tf1 Cells, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tube furnace  (Carbolite Gero)
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Carbolite Gero tube furnace
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
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furnace  (Carbolite Gero)
96
Carbolite Gero furnace
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Furnace, supplied by Carbolite Gero, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell phone video camera sony® cyber-shot dsc-tf1  (Sony)
90
Sony cell phone video camera sony® cyber-shot dsc-tf1
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Cell Phone Video Camera Sony® Cyber Shot Dsc Tf1, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tf1 cnt  (OCSiAl Ltd)
90
OCSiAl Ltd tf1 cnt
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Tf1 Cnt, supplied by OCSiAl Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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coup tf1 nr2f1  (Perseus Proteomics)
86
Perseus Proteomics coup tf1 nr2f1
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Coup Tf1 Nr2f1, supplied by Perseus Proteomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nemod-tf2  (Glycotope GmbH)
90
Glycotope GmbH nemod-tf2
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Nemod Tf2, supplied by Glycotope GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tf-1 cells  (CLS Cell Lines Service GmbH)
93
CLS Cell Lines Service GmbH tf-1 cells
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Tf 1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ir blocking tf1 filter  (Thorlabs)
86
Thorlabs ir blocking tf1 filter
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Ir Blocking Tf1 Filter, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tf1 cell line  (Myelo Therapeutics GmbH)
90
Myelo Therapeutics GmbH tf1 cell line
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
Tf1 Cell Line, supplied by Myelo Therapeutics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tf1.5 core materials  (Lantor GmbH)
90
Lantor GmbH tf1.5 core materials
Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) <t>TF1</t> cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.
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Image Search Results


Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) TF1 cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.

Journal: Biochemical pharmacology

Article Title: Tumor necrosis factor α-mediated inhibition of erythropoiesis involves GATA-1/GATA-2 balance impairment and PU.1 over-expression.

doi: 10.1016/j.bcp.2011.03.030

Figure Lengend Snippet: Fig. 3. Effect of TNFa on GATA-1 transcriptional activity (A) TF1 cells were co- transfected with the GATA-1-expressing vector, pXM-GATA1 and the reporter luciferase plasmids, pGL3-GATA-Luc and pHRG-TK. Transfected cells were treated for 2 h with TNFa or left untreated prior to analysis of luciferase activity. Firefly activity was normalized to Renilla, and the results are expressed as a ratio in arbitrary units. The data are presented as mean S.D. of three independent experiments (**P 0.01 and ***P < 0.001). (B) GATA-1 protein expression in TF1 cells cultured in the presence of GM-CSF (GM) or GM-CSF and TNFa (GM/TNF) was assessed by western blot analysis. Nuclear extracts from human erythroleukemia HEL cells were used as a positive control. b actin was used as an internal control.

Article Snippet: TF1 cells were provided by DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen; Braunschweig, Germany).

Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Luciferase, Cell Culture, Western Blot, Positive Control, Control